Conformation Types of Ubiquitin [M+8H]<sup>8+</sup> Ions from Water:Methanol Solutions: Evidence for the N and A States in Aqueous Solution

Condensed Matter journal club

Conformation Types of Ubiquitin [M+8H]<sup>8+</sup> Ions from Water:Methanol Solutions: Evidence for the N and A States in Aqueous Solution

  • Event time: 11:30am
  • Event date: 20th April 2012
  • Speaker: Jason Kalapothakis (Formerly School of Physics & Astronomy, University of Edinburgh)
  • Location: Room 2511,

Event details

Abstract

Ion mobility and mass spectrometry measurements are used to examine the gas-phase populations of [M+8H]8+ ubiquitin ions formed upon electrospraying 20 different solutions from 100:0 to 5:95 water:methanol that are maintained at pH = 2.0. Over this range of solution conditions, mobility distributions for the +8 charge state show substantial variations. Here we develop a model that treats the combined measurements as one data set. By varying the relative abundances of a discrete set of conformation types, it is possible to represent distributions obtained from any solution. For solutions that favor the well-known A-state ubiquitin, it is possible to represent the gas-phase distributions with seven conformation types. Aqueous conditions that favor the native structure require four more structural types to represent the distribution. This analysis provides the first direct evidence for trace amounts of the A state under native conditions. The method of analysis presented here should help illuminate how solution populations evolve into new gas-phase structures as solvent is removed. Evidence for trace quantities of previously unknown states under native solution conditions may provide insight about the relationship of dynamics to protein function as well as misfolding and aggregation phenomena.
The Journal Of Physical Chemistry B 116 pages 3344-3352 (2012)
pdf version of paper

Authors

Huilin Shi, Nicholas A. Pierson, Stephen J. Valentine, and David E. Clemmer