Microfluidic devices: surface modification and analysis
- Event time: 1:00pm
- Event date: 30th June 2008
- Speaker: Sally McArthur (University of Sheffield)
- Location: Room 2511, James Clerk Maxwell Building (JCMB) James Clerk Maxwell Building Peter Guthrie Tait Road Edinburgh EH9 3FD GB
There are significant limitations inherent in the current technologies used in protein extraction and separation for proteomes larger than 3,000 as this represents the upper limit of protein separation using conventional 2D gels. High-throughput microfluidic shotgun analysis for complex organisms aims to investigate novel micro-channel processing schemes that will enable spatio-temporal separation of both soluble and insoluble proteins that can subsequently be interfaced with mass spectrometry analysis. The ability to control interactions between biomolecules and micro-channel surfaces is an integral component of this project. This talk will discuss the range of polymeric surface modification techniques currently being investigated in order to control protein/surface interactions, enhance protein separation and influence the flow of fluids within microfluidic channels.
In order to understand how the surface properties of a material influence the organization of biomolecules detailed characterization is required. Surface analytical techniques such as X-ray photoelectron spectroscopy (XPS), static secondary ion mass spectrometry (SIMS), and biological assays provide a complementary approach for characterizing these complex systems.
This is a weekly series of informal talks given primarily by members of the soft condensed matter and statistical mechanics groups, but is also open to members of other groups and external visitors. The aim of the series is to promote discussion and learning of various topics at a level suitable to the broad background of the group. Everyone is welcome to attend..