Using dielectrophoretic manipulation with nucleic acid sensors
- Event time: 1:00pm
- Event date: 17th October 2011
- Speaker: Mira Nishimura (Formerly School of Physics & Astronomy, University of Edinburgh)
- Location: Room 2511, James Clerk Maxwell Building (JCMB) James Clerk Maxwell Building Peter Guthrie Tait Road Edinburgh EH9 3FD GB
Dielectrophoresis (DEP) arises from the creation of a force acting on an electrically polarized particle when subjected to a non-uniform electric field. Solvated particles can respond to this force by either moving towards (positive DEP) or away (negative DEP) from the electrodes generating the field. The technique can be used to manipulate biological particles including DNA and RNA, opening up new opportunities for nucleic acid based biosensing devices. The aim of the project is to develop a RNA hybridization probe functionalised on a microelectrode, capable of rapid detection and DEP based target sorting. The DEP collection and repulsion of ribosomal RNA on microfabricated interdigitated Au electrodes are observed using total internal reflection (TIRF) microscopy, and measured as a function of the strength and frequency of the applied electric field. The possibility of using quantum dots to increase sensor efficiency is also explored by taking in to consideration the DEP force dependence on particle size and the effect of electrode geometry.
This is a weekly series of informal talks given primarily by members of the soft condensed matter and statistical mechanics groups, but is also open to members of other groups and external visitors. The aim of the series is to promote discussion and learning of various topics at a level suitable to the broad background of the group. Everyone is welcome to attend..