Theoretical and Experimental Dissection of DNA Loop-Mediated Repression

Condensed Matter journal club

Theoretical and Experimental Dissection of DNA Loop-Mediated Repression

  • Event time: 11:30am
  • Event date: 25th January 2013
  • Speaker: (School of Physics & Astronomy, University of Edinburgh)
  • Location: Room 2511,

Event details

Abstract

Transcriptional networks across all domains of life feature a wide range of regulatory architectures. Theoretical models now make clear predictions about how key parameters describing those architectures modulate gene expression, and the ability to construct genetic circuits with tunable parameters enables precise tests of such models. We dissect gene regulation through DNA looping by tuning network parameters such as repressor copy number, DNA binding strengths, and loop length in both thermodynamic models and experiments. Our results help clarify the short-length mechanical properties of DNA.
PRL 110 article 018101 (2013)
pdf version

Authors

James Q. Boedicker, Hernan G. Garcia, Rob Phillips