Cryo FIB-SEM

We are delighted to introduce a new electron microscope facility available to all researchers across the university. We also welcome external users from other universities and industry. This Zeiss Crossbeam 550 FIB-SEM with cryogenic attachment from Quorum Technologies is one of only a few such instruments in the UK and offers a multitude of powerful techniques to extract as much information as possible from a large range of samples. The microscope offers resolution down to ~1nm, elemental contrast and identification, fast and accurate milling and patterning using the focussed ion beam (FIB), nanoscale tomography, TEM sample preparation and the ability to work on liquids, gels, polymers, biological matter and many other materials using the optional cryo mode.

The university logo milled into a piece of silicon.

The microscope has the following:

  • Resolution of 0.9nm at 15kV and 1.6nm at 1kV using the FEG electron column
  • FIB imaging and milling with 3nm resolution at 30kV
  • Automated serial slice and imaging for destructive nanotomography
  • Automated TEM sample preparation
  • Automated stitching of images to give high field of few, high resolution images
  • Oxford Instruments X-Max 150 EDS detector
  • Oxford Instruments Omniprobe 200 micromanipulator
  • Gas insertion system for deposition of platinum or carbon, or for gas assisted etching
  • In-lens, SESI, ESB and Backscatter detectors
  • Quorum Technologies¬† PP3010T Cryo-SEM preparation system
  • STEM detector
  • Charge compensator
  • Flood gun
  • Plasma cleaner

Members of the university will be charged a rate of £50/hr. External users have a higher rate. For further information on capabilities, feasibilities, fees, training, access and more please contact the instrument scientist Thomas Glen at t.glen [at] ed.ac.uk

Also available in the lab:

  • Korvus HEX thin film deposition system
  • Leica HPM100 high pressure freezer
  • Leica AFS2 freeze substitution
  • Dedicated data PC with Avizo software

Example images are available in the gallery below: