Architectural Transitions in Vibrio Cholerae Biofilms At Single-Cell Resolution

Condensed Matter journal club

Architectural Transitions in Vibrio Cholerae Biofilms At Single-Cell Resolution

  • Event time: 11:30am until 12:30pm
  • Event date: 16th February 2018
  • Speaker: (School of Physics & Astronomy, University of Edinburgh)
  • Location: 2511

Event details

Many bacterial species colonize surfaces and form dense 3D structures, known as biofilms, which are highly tolerant to antibiotics and constitute one of the major forms of bacterial biomass on Earth.  Bacterial biofilms display remarkable changes during their development from initial attachment to maturity, yet the cellular architecture that gives rise to collective biofilm morphology during growth is largely unknown. Here, we use high-resolution optical microscopy to image all individual cells in Vibrio cholerae biofilms at different stages of development, including colonies that range in size from 2 to 4,500 cells. From these data, we extracted the precise 3D cellular arrangements, cell shapes, sizes, and global morphological features during biofilm growth on submerged glass substrates under flow. We discovered several critical transitions of the internal and external biofilm architectures that separate the major phases of V. cholerae biofilm growth. Optical imaging of biofilms with single-cell resolution provides a new window into biofilm formation that will prove invaluable to understanding the mechanics underlying biofilm development.

Event resources

About Condensed Matter journal club

Given the diversity of research in the CM group, chosen topics vary widely. We tend to stick to high-impact journals - Nature, Science, PNAS and PRL have been popular - but this is not prescriptive..

Find out more about Condensed Matter journal club.