Physiological model of the mechanism of action of DNA targeting antibiotic: Stochastic simulations of the growth-inhibition curve
Physiological model of the mechanism of action of DNA targeting antibiotic: Stochastic simulations of the growth-inhibition curve
- Event time: 1:00pm until 2:00pm
- Event date: 26th February 2018
- Speaker: Nikola Ojkic (Formerly School of Physics & Astronomy, University of Edinburgh)
- Location: Room 2511, James Clerk Maxwell Building (JCMB) James Clerk Maxwell Building Peter Guthrie Tait Road Edinburgh EH9 3FD GB
Event details
Combatting the emergence of antibiotic resistance requires better understanding of antibiotic mechanism of action as well as the mechanisms by which resistance evolves. One of the most potent DNA targeting antibiotics in clinical use is ciprofloxacin. Even though it is known that ciprofloxacin causes lethal DNA breaks, the mechanistic model that will bridge single-cell level antibiotic physiology to the population level dynamics is still missing. In this talk I will present a model of replication fork inhibition and DNA double strand breakage by ciprofloxacin. Stochastic simulations of this model reproduce the shapes of experimentally measured growth-inhibition curves and predict their growth-medium dependence. My findings are also supported by live cell imaging of bacteria grown in the presence of the antibiotic.
About Condensed Matter lunchtime seminars
This is a weekly series of informal talks given primarily by members of the institute of condensed matter and complex systems, but is also open to members of other groups and external visitors. The aim of the series is to promote discussion and learning of various topics at a level suitable to the broad background of the group. Everyone is welcome to attend..