Membrane Sculpting By Curved DNA Origami Scaffolds

Condensed Matter journal club

Membrane Sculpting By Curved DNA Origami Scaffolds

  • Event time: 11:30am until 12:30pm
  • Event date: 25th May 2018
  • Speaker: (School of Physics & Astronomy, University of Edinburgh)
  • Location: Room 2511,

Event details

Membrane sculpting and transformation is essential for many cellular functions, thus being largely regulated
by self-assembling and self-organizing protein coats. Their functionality is often encoded by particular
spatial structures. Prominent examples are BAR domain proteins, the 'banana-like' shapes of which are thought
to aid scaffolding and membrane tubulation. To elucidate whether 3D structure can be uncoupled from other
functional features of complex scaffolding proteins, we hereby develop curved DNA origami in various shapes
and stacking features, following the presumable design features of BAR proteins, and characterize their
ability for membrane binding and transformation. We show that dependent on curvature, membrane affinity and
surface density, DNA origami coats can indeed reproduce the activity of membrane-sculpting proteins such as
BAR, suggesting exciting perspectives for using them in bottom-up approaches towards minimal biomimetic
cellular machineries.
 

Event resources

About Condensed Matter journal club

Given the diversity of research in the CM group, chosen topics vary widely. We tend to stick to high-impact journals - Nature, Science, PNAS and PRL have been popular - but this is not prescriptive..

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