Differential dynamic microscopy for in situ characterization of nanoparticles in biological environments

Condensed Matter lunchtime seminar

Differential dynamic microscopy for in situ characterization of nanoparticles in biological environments

  • Event time: 1:00pm until 2:00pm
  • Event date: 29th November 2021
  • Speaker: Marine Le Goas (Université de Montréal)
  • Location: TBC

Event details

In both nanomedicine and nanotoxicology fields, understanding the interaction of nanoparticles with biological systems is key. Yet, despite several years of research, the biological behavior of nanoparticles is still hard to understand and predict. One main challenge for these studies remains the characterization of the particles in situ, i.e., in a real biological environment. The complexity and dynamic characteristics of biological environments are indeed not compatible with most current characterization techniques.

We will show how differential dynamic microscopy (DDM) can provide valuable in situ information regarding nanoparticles’ biological behavior. The technique was first used to investigate the diffusion of isotropic vs anisotropic polymeric particles in gels mimicking the extracellular matrix. We also developed a new theoretical framework using DDM to quantify protein adsorption while simultaneously measuring particle size aggregation and the aggregates fractal dimension. Lastly, we plan to apply the new analysis scheme called “flow-DDM” (which enables particle sizing in flowing conditions) to in vivo conditions, using zebrafish larvae as model organisms. Our results highlight the potential of the DDM technique to characterize nanoparticles under realistic biological conditions.

About Condensed Matter lunchtime seminars

This is a weekly series of informal talks given primarily by members of the soft condensed matter and statistical mechanics groups, but is also open to members of other groups and external visitors. The aim of the series is to promote discussion and learning of various topics at a level suitable to the broad background of the group. Everyone is welcome to attend..

Find out more about Condensed Matter lunchtime seminars.